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pgem-easy vector cloning kit  (Promega)

 
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    Promega pgem-easy vector cloning kit
    Pgem Easy Vector Cloning Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgem+-t+easy-shuttle+vector/pgem+t+easy/10__1111_slash_boj__12415-92-6-5
    Average 90 stars, based on 1 article reviews
    pgem-easy vector cloning kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Functional Analysis of Nicotine Demethylase Genes Reveals Insights into the Evolution of Modern Tobacco
    Article Snippet: .. PCR products were visualized on an ethidium bromide-stained 1% agarose gel and directly cloned into the pGEM Easy cloning vector (Promega Corp., Madison, WI). ..

    Article Title: The transcription factor EIN3 is constitutively expressed in miniature roses with differences in postharvest life
    Article Snippet: The transcription factor EIN3 is constitutively expressed in miniature roses with differences in postharvest life Renate Müller, Carolyn Owen, Zhong-Tian Xue, Margaretha Welander & Bjarne Stummann To cite this article: Renate Müller, Carolyn Owen, Zhong-Tian Xue, Margaretha Welander & Bjarne Stummann (2003) The transcription factor EIN3 is constitutively expressed in miniature roses with differences in postharvest life, The Journal of Horticultural Science and Biotechnology, 78:1, 10-14, DOI: 10.1080/14620316.2003.11511575 To link to this article: http://dx.doi.org/10.1080/14620316.2003.11511575

    Agarose Gel Electrophoresis:

    Article Title: Functional Analysis of Nicotine Demethylase Genes Reveals Insights into the Evolution of Modern Tobacco
    Article Snippet: .. PCR products were visualized on an ethidium bromide-stained 1% agarose gel and directly cloned into the pGEM Easy cloning vector (Promega Corp., Madison, WI). ..

    Clone Assay:

    Article Title: Functional Analysis of Nicotine Demethylase Genes Reveals Insights into the Evolution of Modern Tobacco
    Article Snippet: .. PCR products were visualized on an ethidium bromide-stained 1% agarose gel and directly cloned into the pGEM Easy cloning vector (Promega Corp., Madison, WI). ..

    Article Title: The transcription factor EIN3 is constitutively expressed in miniature roses with differences in postharvest life
    Article Snippet: The transcription factor EIN3 is constitutively expressed in miniature roses with differences in postharvest life Renate Müller, Carolyn Owen, Zhong-Tian Xue, Margaretha Welander & Bjarne Stummann To cite this article: Renate Müller, Carolyn Owen, Zhong-Tian Xue, Margaretha Welander & Bjarne Stummann (2003) The transcription factor EIN3 is constitutively expressed in miniature roses with differences in postharvest life, The Journal of Horticultural Science and Biotechnology, 78:1, 10-14, DOI: 10.1080/14620316.2003.11511575 To link to this article: http://dx.doi.org/10.1080/14620316.2003.11511575

    Cloning:

    Article Title: Functional Analysis of Nicotine Demethylase Genes Reveals Insights into the Evolution of Modern Tobacco
    Article Snippet: .. PCR products were visualized on an ethidium bromide-stained 1% agarose gel and directly cloned into the pGEM Easy cloning vector (Promega Corp., Madison, WI). ..

    Article Title: Expression in yeast of secreted lignin peroxidase with improved 2,4-dichlorophenol degradability by DNA shuffling.
    Article Snippet: Lignin peroxidase (LiP) fro aromatic compounds and problem of the wild type matic compounds.. We app method to obtain mutant and they were successfull resulting variants showed activity and stability again toward 2,4-DCP and H2O2 Amino acid sequence anal near the surface or Ca2+ b

    Article Title: Generation and characterization of a recombinant chimeric protein (rCpLi) consisting of B-cell epitopes of a dermonecrotic protein from Loxosceles intermedia spider venom.
    Article Snippet: .. The double stranded DNA was subcloned into he easy cloning vector pGEM (Promega) and subsequently treated ith restriction enzymes NcoI and XhoI. .. The DNA fragment was urified and cloned into the expression vector pET 26b (Stratagene), reviously treated with NcoI and XhoI and alkaline phosphatase.

    Article Title: The transcription factor EIN3 is constitutively expressed in miniature roses with differences in postharvest life
    Article Snippet: The transcription factor EIN3 is constitutively expressed in miniature roses with differences in postharvest life Renate Müller, Carolyn Owen, Zhong-Tian Xue, Margaretha Welander & Bjarne Stummann To cite this article: Renate Müller, Carolyn Owen, Zhong-Tian Xue, Margaretha Welander & Bjarne Stummann (2003) The transcription factor EIN3 is constitutively expressed in miniature roses with differences in postharvest life, The Journal of Horticultural Science and Biotechnology, 78:1, 10-14, DOI: 10.1080/14620316.2003.11511575 To link to this article: http://dx.doi.org/10.1080/14620316.2003.11511575

    Plasmid Preparation:

    Article Title: Functional Analysis of Nicotine Demethylase Genes Reveals Insights into the Evolution of Modern Tobacco
    Article Snippet: .. PCR products were visualized on an ethidium bromide-stained 1% agarose gel and directly cloned into the pGEM Easy cloning vector (Promega Corp., Madison, WI). ..

    Article Title: Expression in yeast of secreted lignin peroxidase with improved 2,4-dichlorophenol degradability by DNA shuffling.
    Article Snippet: Lignin peroxidase (LiP) fro aromatic compounds and problem of the wild type matic compounds.. We app method to obtain mutant and they were successfull resulting variants showed activity and stability again toward 2,4-DCP and H2O2 Amino acid sequence anal near the surface or Ca2+ b

    Article Title: Generation and characterization of a recombinant chimeric protein (rCpLi) consisting of B-cell epitopes of a dermonecrotic protein from Loxosceles intermedia spider venom.
    Article Snippet: .. The double stranded DNA was subcloned into he easy cloning vector pGEM (Promega) and subsequently treated ith restriction enzymes NcoI and XhoI. .. The DNA fragment was urified and cloned into the expression vector pET 26b (Stratagene), reviously treated with NcoI and XhoI and alkaline phosphatase.

    Article Title: The transcription factor EIN3 is constitutively expressed in miniature roses with differences in postharvest life
    Article Snippet: The transcription factor EIN3 is constitutively expressed in miniature roses with differences in postharvest life Renate Müller, Carolyn Owen, Zhong-Tian Xue, Margaretha Welander & Bjarne Stummann To cite this article: Renate Müller, Carolyn Owen, Zhong-Tian Xue, Margaretha Welander & Bjarne Stummann (2003) The transcription factor EIN3 is constitutively expressed in miniature roses with differences in postharvest life, The Journal of Horticultural Science and Biotechnology, 78:1, 10-14, DOI: 10.1080/14620316.2003.11511575 To link to this article: http://dx.doi.org/10.1080/14620316.2003.11511575

    other:

    Article Title: Changes in cardiac heparan sulfate proteoglycan expression and streptozotocin-induced diastolic dysfunction in rats
    Article Snippet: The PCR products were purified with a Concert Rapid Gel Extraction System (Invitrogen) and cloned with a pGEM-Easy cloning vector (Promega, Madison, WI, USA.).

    Article Title: Genetic mapping of the apospory-specific genomic region in Pennisetum squamulatum using retrotransposon-based molecular markers.
    Article Snippet: Pennisetum squamulatum reproduces by apomixis, a type of asexual reproduction through seeds.. Apomixis in P. squamulatum is transmitted as a dominant Mendelian trait, and a genomic region, the apospory-speciWc genomic region (ASGR), is suYcient for inheritance of the trait.. The ASGR is physically large (>50 Mb), highly heterochromatic, hemizygous, and recombinationally suppressed.

    Article Title: Microfluidic platforms for rapid screening of cancer affinity reagents by using tissue samples
    Article Snippet: The amplified 95-bp PCR products were cloned with a pGEM ® easy TA cloning vector (Promega), transformed into E. coli (TOP10 strain) cells, and grown on LB-agar plates containing 100 μ g/ml of ampicillin (Sigma-Aldrich).

    Article Title: Narrow water barriers prevent multiple colonizations and limit gene flow among California Channel Island wild buckwheats (Eriogonum: Polygonaceae)
    Article Snippet: All LFY amplicons were cloned (Promega PGEM-easy vector cloning kit, following the manufacturer’s instructions in 1/4 reactions) and one clone per individual was sequenced.

    Sequencing:

    Article Title: The transcription factor EIN3 is constitutively expressed in miniature roses with differences in postharvest life
    Article Snippet: The transcription factor EIN3 is constitutively expressed in miniature roses with differences in postharvest life Renate Müller, Carolyn Owen, Zhong-Tian Xue, Margaretha Welander & Bjarne Stummann To cite this article: Renate Müller, Carolyn Owen, Zhong-Tian Xue, Margaretha Welander & Bjarne Stummann (2003) The transcription factor EIN3 is constitutively expressed in miniature roses with differences in postharvest life, The Journal of Horticultural Science and Biotechnology, 78:1, 10-14, DOI: 10.1080/14620316.2003.11511575 To link to this article: http://dx.doi.org/10.1080/14620316.2003.11511575



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